356.

Kim II. K, Benevenga NJ, Grummer RH. Estimation of the fraction of the lactose in a high lactose diet available for fermentation in the cecum and colon of the rat. J Nutr. 1978; 108(1):79-89. (CA) [Expts. were conducted to det. the amt. of lactose which passed into the large intestine and the lactase activity in the small intestine of rats (200 g) fed a control diet or a diet contg. 30% lactose. The fraction of lactose consumed in a single 1-h meal that escaped hydrolysis in the small intestine was est. by measuring the area under a smooth curve of a plot of lactose:marker ratio in the terminal ileum expressed as a fraction of intake, against the fraction of the total marker that passed into the large intestine. This amounted to ~30% and ~31.5% of the lactose consumed for the rats fed the control and 30% lactose diets, resp. In another expt. in which the rats were fed a diet contg. 30% lactose and Cr-EDTA marker ad libitum for 1 wk, ~43% of the lactose consumed became available for fermn. in the large intestine. Evidently, a substantial fraction of the lactose ingested is available for fermn. in the large intestine. The lactase activity of homogenates of the small intestine of rats fed the control or high lactose diet was 106 or 115 mg lactose/30 min/rat, resp. The lactase activity in the small intestine homogenate (mucosa + contents) was higher than that actually available in vivo (110 vs. 69 mg/60 min/rat).

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